Coding

Part:BBa_K1382001:Design

Designed by: Maryam Ige, Janki Patel   Group: iGEM14_Stony_Brook   (2014-06-23)


GST-melittin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85


Design Notes

Thrombin was the initial cleavage site, however we decided to use TEV because it was more efficient. As a result, we have both thrombin and TEV cleavage sites.


Source

Melittin- Apis Mellifera


References